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  • fadista
    Member
    • Sep 2008
    • 37

    #1

    MAQ and indel detection

    I am using solexa paired-end data and after I mapped my reads to a reference sample with the MAQ software a message appears on the screen:
    "[maq_indel_pe] the indel detector only works with short-insert mate-pair reads".
    I am working with fragment sizes that have an average length of 315 bp and in the parameters -a and -A of the 'match' function I put the number 350.

    My question is what does this message mean and am I not able to detect indels and, if not, why is that?


    Best regards,
    João Fadista
  • zee
    NGS specialist
    • Apr 2008
    • 249

    #2
    Hi.

    I've seen that message with single-end read alignments i.e.

    maq out.map chr.bfa read1.bfq

    So perhaps it's assuming that you didnt submit the other read2.bfq mate-pair file.

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