Seqanswers Leaderboard Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • arkilis
    Senior Member
    • Jul 2013
    • 119

    dna, dna_sm and dna_rm which one should I use to form a blast database?

    I saw there are a couple of files on the ensembl ftp website:

    ftp://ftp.ensembl.org/pub/release-76...lanoleuca/dna/

    * 'dna' - unmasked genomic DNA sequences.

    * 'dna_rm' - masked genomic DNA. Interspersed repeats and low
    complexity regions are detected with the RepeatMasker tool and masked
    by replacing repeats with 'N's.

    * 'dna_sm' - soft-masked genomic DNA. All repeats and low complexity regions
    have been replaced with lowercased versions of their nucleic base

    Still don't know which file should I downloaded to format a blast database? Thanks!
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    What are you going to do using the blast database you create?

    Comment

    • arkilis
      Senior Member
      • Jul 2013
      • 119

      #3
      Originally posted by GenoMax View Post
      What are you going to do using the blast database you create?
      Just normal blast, might want to know which species belongs to for those samples I already sequenced.

      Thanks for your reply.

      Comment

      • GenoMax
        Senior Member
        • Feb 2008
        • 7142

        #4
        Get the unmasked DNA for a normal blast.

        Comment

        Latest Articles

        Collapse

        • seqadmin
          Pathogen Surveillance with Advanced Genomic Tools
          by seqadmin




          The COVID-19 pandemic highlighted the need for proactive pathogen surveillance systems. As ongoing threats like avian influenza and newly emerging infections continue to pose risks, researchers are working to improve how quickly and accurately pathogens can be identified and tracked. In a recent SEQanswers webinar, two experts discussed how next-generation sequencing (NGS) and machine learning are shaping efforts to monitor viral variation and trace the origins of infectious...
          Yesterday, 11:48 AM
        • seqadmin
          New Genomics Tools and Methods Shared at AGBT 2025
          by seqadmin


          This year’s Advances in Genome Biology and Technology (AGBT) General Meeting commemorated the 25th anniversary of the event at its original venue on Marco Island, Florida. While this year’s event didn’t include high-profile musical performances, the industry announcements and cutting-edge research still drew the attention of leading scientists.

          The Headliner
          The biggest announcement was Roche stepping back into the sequencing platform market. In the years since...
          03-03-2025, 01:39 PM
        • seqadmin
          Investigating the Gut Microbiome Through Diet and Spatial Biology
          by seqadmin




          The human gut contains trillions of microorganisms that impact digestion, immune functions, and overall health1. Despite major breakthroughs, we’re only beginning to understand the full extent of the microbiome’s influence on health and disease. Advances in next-generation sequencing and spatial biology have opened new windows into this complex environment, yet many questions remain. This article highlights two recent studies exploring how diet influences microbial...
          02-24-2025, 06:31 AM

        ad_right_rmr

        Collapse

        News

        Collapse

        Topics Statistics Last Post
        Started by seqadmin, 03-20-2025, 05:03 AM
        0 responses
        34 views
        0 reactions
        Last Post seqadmin  
        Started by seqadmin, 03-19-2025, 07:27 AM
        0 responses
        42 views
        0 reactions
        Last Post seqadmin  
        Started by seqadmin, 03-18-2025, 12:50 PM
        0 responses
        35 views
        0 reactions
        Last Post seqadmin  
        Started by seqadmin, 03-03-2025, 01:15 PM
        0 responses
        190 views
        0 reactions
        Last Post seqadmin  
        Working...