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miRNA analysis
Hello all,
I am a newbie at bioinformatics and am trying to analyse some miRNA data from the SOLiD sequencer I.e. color space csfasta format. I think I have managed to align these to pre-miRNA and mature sequences to produce SAM files.
My question is what to do next? I need to now count the frequencies of each miRNAs and carry out normalisation so these are relative frequencies from each sample??? How can I achieve this and is there then a nice way to present this data? Or am I barking up the wrong tree and if so what do you advise??
I would be very appreciative of any advice from more experienced bioinformaticians.
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...07-08-2024, 03:19 PM
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