linkage analysis in targeted NGS data

[lsantome]

hi all,

tipically the aim of linkage analysis in human disease is to detect candiate genes based upon cosegregation of SNP markers and the familial phenotype.

but, what if I have the candidate gene (and mutation) and I want to quantify its association with the trait? can I use the same approach?

I'm working with targeted NGS data (gene pannels) and I want to demonstrate the association between a candidate variant and a phenotype not previously reported.

Any suggestion is welcome

Thank you in advance!