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  • shashankgupta
    Member
    • Feb 2015
    • 35

    #1

    Protein sequence analysis

    Hi,

    I have a multiple protein sequence which looks like -


    >gnl|TC-DB|198412035 1.A.70.1.3 PREDICTED: hypothetical protein [Ciona intestinalis]
    MDQKIVFILLLVVLLVSQATADDGWVRTGLAVARLVVGRRRRRWNEANGLEKLSSDAEETLSAAEMEEVMQKIMDHQ
    >gnl|TC-DB|198415263 1.A.70.1.2 PREDICTED: hypothetical protein [Ciona intestinalis]
    MDRKIAFAFLFVILQVTTVSAGWGSLLRVETKAILGTLALRRRTWNENKASQQITPEMEEKLDAEMEKLMQQLAEDQQ
    >gnl|TC-DB|210060745 2.A.39.3.6 Chain A, Structure Of Mhp1, A Nucleobase-Cation-Symport-1 Family Transporter
    MNSTPIEEARSLLNPSNAPTRYAERSVGPFSLAAIWFAMAIQVAIFIAAGQMTSSFQVWQVIVAIAAGCTIAVILLFFTQ
    SAAIRWGINFTVAARMPFGIRGSLIPITLKALLSLFWFGFQTWLGALALDEITRLLTGFTNLPLWIVIFGAIQVVTTFYG
    ITFIRWMNVFASPVLLAMGVYMVYLMLDGADVSLGEVMSMGGENPGMPFSTAIMIFVGGWIAVVVSIHDIVKECKVDP
    SREGQTKADARYATAQWLGMVPASIIFGFIGAASMVLVGEWNPVIAITEVVGGVSIPMAILFQVFVLLATWSTNPAANL
    SPAYTLCSTFPRVFTFKTGVIVSAVVGLLMMPWQFAGVLNTFLNLLASALGPLAGIMISDYFLVRRRRISLHDLYRTKGI
    >gnl|TC-DB|58177374 1.A.2.2.2 Chain B, Intermediate Gating Structure 2 Of The Inwardly Rectifying K+ Channel Kirbac3.1
    MTGGMKPPARKPRILNSDGSSNITRLGLEKRGWLDDHYHDLLTVSWPVFITLITGLYLVTNALFALAYLACGDVIENARP
    GSFTDAFFFSVQTMATIGYGKLIPIGPLANTLVTLEALCGMLGLAVAASLIYARFTRPTAGVLFSSRMVISDFEGKPTLM
    MRLANLRIEQIIEADVHLVLVRSEISQEGMVFRRFHDLTLTRSRSPIFSLSWTVMHPIDHHSPIYGETDETLRNSHSEFL
    VLFTGHHEAFAQNVHARHAYSCDEIIWGGHFVDVFTTLPDGRRALDLGKFHEIAQHHHHHH
    >gnl|TC-DB|A0CIB0 1.A.17.3.1 Chromosome undetermined scaffold_19, whole genome shotgun sequence OS=Paramecium tetraurelia GN=GSPATT00007662001 PE=4 SV=1
    MDDQNQPILQEQPKPKQKKPLLNTKMVKKQKMQNKKEENLREILNFYTNQVDARKFLQKMKAVVDSNQQEKKYQDD
    NEYNEMQDIYEDYNMGDLVIVFPNPDADGVKNPPITYKEAEKFYDETLAQKNDDVSESQLKKEKEAFLLAFLMLDDYQ

    .

    .

    .

    and so on... (almost around 6000 sequences)

    I need to do functional annotation of it, how can i do that ?

    Best !

    Shashank
  • Medhat
    Member
    • Jun 2013
    • 37

    #2
    Blast2Go a simple yet powerful tool to make Blast,Mapping,Annotation


    Or

    using pfam to search for protein with hmmer
    ftp://ftp.sanger.ac.uk/pub/databases/Pfam/Tools/
    Last edited by Medhat; 05-21-2015, 11:10 PM.

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