Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Protein sequence analysis

    Hi,

    I have a multiple protein sequence which looks like -


    >gnl|TC-DB|198412035 1.A.70.1.3 PREDICTED: hypothetical protein [Ciona intestinalis]
    MDQKIVFILLLVVLLVSQATADDGWVRTGLAVARLVVGRRRRRWNEANGLEKLSSDAEETLSAAEMEEVMQKIMDHQ
    >gnl|TC-DB|198415263 1.A.70.1.2 PREDICTED: hypothetical protein [Ciona intestinalis]
    MDRKIAFAFLFVILQVTTVSAGWGSLLRVETKAILGTLALRRRTWNENKASQQITPEMEEKLDAEMEKLMQQLAEDQQ
    >gnl|TC-DB|210060745 2.A.39.3.6 Chain A, Structure Of Mhp1, A Nucleobase-Cation-Symport-1 Family Transporter
    MNSTPIEEARSLLNPSNAPTRYAERSVGPFSLAAIWFAMAIQVAIFIAAGQMTSSFQVWQVIVAIAAGCTIAVILLFFTQ
    SAAIRWGINFTVAARMPFGIRGSLIPITLKALLSLFWFGFQTWLGALALDEITRLLTGFTNLPLWIVIFGAIQVVTTFYG
    ITFIRWMNVFASPVLLAMGVYMVYLMLDGADVSLGEVMSMGGENPGMPFSTAIMIFVGGWIAVVVSIHDIVKECKVDP
    SREGQTKADARYATAQWLGMVPASIIFGFIGAASMVLVGEWNPVIAITEVVGGVSIPMAILFQVFVLLATWSTNPAANL
    SPAYTLCSTFPRVFTFKTGVIVSAVVGLLMMPWQFAGVLNTFLNLLASALGPLAGIMISDYFLVRRRRISLHDLYRTKGI
    >gnl|TC-DB|58177374 1.A.2.2.2 Chain B, Intermediate Gating Structure 2 Of The Inwardly Rectifying K+ Channel Kirbac3.1
    MTGGMKPPARKPRILNSDGSSNITRLGLEKRGWLDDHYHDLLTVSWPVFITLITGLYLVTNALFALAYLACGDVIENARP
    GSFTDAFFFSVQTMATIGYGKLIPIGPLANTLVTLEALCGMLGLAVAASLIYARFTRPTAGVLFSSRMVISDFEGKPTLM
    MRLANLRIEQIIEADVHLVLVRSEISQEGMVFRRFHDLTLTRSRSPIFSLSWTVMHPIDHHSPIYGETDETLRNSHSEFL
    VLFTGHHEAFAQNVHARHAYSCDEIIWGGHFVDVFTTLPDGRRALDLGKFHEIAQHHHHHH
    >gnl|TC-DB|A0CIB0 1.A.17.3.1 Chromosome undetermined scaffold_19, whole genome shotgun sequence OS=Paramecium tetraurelia GN=GSPATT00007662001 PE=4 SV=1
    MDDQNQPILQEQPKPKQKKPLLNTKMVKKQKMQNKKEENLREILNFYTNQVDARKFLQKMKAVVDSNQQEKKYQDD
    NEYNEMQDIYEDYNMGDLVIVFPNPDADGVKNPPITYKEAEKFYDETLAQKNDDVSESQLKKEKEAFLLAFLMLDDYQ

    .

    .

    .

    and so on... (almost around 6000 sequences)

    I need to do functional annotation of it, how can i do that ?

    Best !

    Shashank

  • #2
    Blast2Go a simple yet powerful tool to make Blast,Mapping,Annotation


    Or

    using pfam to search for protein with hmmer
    ftp://ftp.sanger.ac.uk/pub/databases/Pfam/Tools/
    Last edited by Medhat; 05-21-2015, 11:10 PM.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Essential Discoveries and Tools in Epitranscriptomics
      by seqadmin




      The field of epigenetics has traditionally concentrated more on DNA and how changes like methylation and phosphorylation of histones impact gene expression and regulation. However, our increased understanding of RNA modifications and their importance in cellular processes has led to a rise in epitranscriptomics research. “Epitranscriptomics brings together the concepts of epigenetics and gene expression,” explained Adrien Leger, PhD, Principal Research Scientist...
      04-22-2024, 07:01 AM
    • seqadmin
      Current Approaches to Protein Sequencing
      by seqadmin


      Proteins are often described as the workhorses of the cell, and identifying their sequences is key to understanding their role in biological processes and disease. Currently, the most common technique used to determine protein sequences is mass spectrometry. While still a valuable tool, mass spectrometry faces several limitations and requires a highly experienced scientist familiar with the equipment to operate it. Additionally, other proteomic methods, like affinity assays, are constrained...
      04-04-2024, 04:25 PM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Yesterday, 08:47 AM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-11-2024, 12:08 PM
    0 responses
    60 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 10:19 PM
    0 responses
    59 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 04-10-2024, 09:21 AM
    0 responses
    54 views
    0 likes
    Last Post seqadmin  
    Working...
    X