Authors recommend at least 6 replicates/condition in an RNA-seq experiment, and up to 12 in studies where identifying the majority of differentially expressed genes is critical. They recommend experiments with greater than 12 replicates should use DESeq, while those with fewer than 12 replicates should use edgeR.
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CRISPR/Cas9 sparked the gene editing revolution for both research and therapeutics.1 But this system still showed severe issues that limited its applications. The most prominent were the heavy reliance on PAM sequences, delivery limitations, double-stranded breaks that prompt unintended edits and cell death, and editing inefficiency (both in targeting and in knock-in reliability).
Despite this, “CRISPR helped turn genome editing from a specialized technique into...07-31-2026, 11:01 AM