Seqanswers Leaderboard Ad

Collapse

Announcement

Collapse
No announcement yet.
X
 
  • Filter
  • Time
  • Show
Clear All
new posts

  • Best tool to view sequence alignment?

    I blasted my de novo assembled RNA-Seq transcriptome for a particular protein using tblastn, and found three transcripts similar to this protein. The best hit matches with a 50% percent identity (e value 1E-11).

    I would like to create a figure displaying the sequence alignment with the greatest match to this protein, or all three transcripts that matched. I used ClustalW and Clustal Omega to align the sequences of my transcripts to the mRNA transcripts of the known protein I pulled off of NCBI but I don't like the aesthetics of the output and I'm not sure if this is the best way to do it.

    Any suggestions on a nice way to succinctly display sequence homology between my transcripts and this protein?

  • #2
    Have you looked at tools here: http://www.ebi.ac.uk/Tools/msa/

    You can also try MEGA (http://www.megasoftware.net/).

    The 50% identity over how many AA? You may only be looking at a similar/identical domain.

    Comment

    Latest Articles

    Collapse

    • seqadmin
      Understanding Genetic Influence on Infectious Disease
      by seqadmin




      During the COVID-19 pandemic, scientists observed that while some individuals experienced severe illness when infected with SARS-CoV-2, others were barely affected. These disparities left researchers and clinicians wondering what causes the wide variations in response to viral infections and what role genetics plays.

      Jean-Laurent Casanova, M.D., Ph.D., Professor at Rockefeller University, is a leading expert in this crossover between genetics and infectious...
      09-09-2024, 10:59 AM
    • seqadmin
      Addressing Off-Target Effects in CRISPR Technologies
      by seqadmin






      The first FDA-approved CRISPR-based therapy marked the transition of therapeutic gene editing from a dream to reality1. CRISPR technologies have streamlined gene editing, and CRISPR screens have become an important approach for identifying genes involved in disease processes2. This technique introduces targeted mutations across numerous genes, enabling large-scale identification of gene functions, interactions, and pathways3. Identifying the full range...
      08-27-2024, 04:44 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by seqadmin, Today, 06:25 AM
    0 responses
    13 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, Yesterday, 01:02 PM
    0 responses
    12 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 09-18-2024, 06:39 AM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Started by seqadmin, 09-11-2024, 02:44 PM
    0 responses
    14 views
    0 likes
    Last Post seqadmin  
    Working...
    X