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  • Anelda
    Member
    • May 2010
    • 30
    #1

    Normalizing microRNA seq data

    Hi there,

    I'm trying to find out what is an acceptable way of normalizing microRNA deep sequencing data.

    We have a number of samples (biological replicates). I would like to see which known micrornas are expressed in these. We are also running more experiments under various conditions and I'd like to compare these with the baseline experiments.

    I have found two methods - TPM and dividing by the total number of reads in the library.

    Does anyone have ideas/experience with this?

    Thanks!
    Tags: microrna, normalize
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