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Hi,
I've been using FeatureCounts on differentially accessible regions between multiple atac-seq samples to get the reads per region in each sample. My goal is to make a heatmap to visually represent the change in accessibility. As each sample wasn't sequenced to the same depth and the number of mapped reads isn't equal, how can I normalize the FeatureCounts results for each sample?
I've been using FeatureCounts on differentially accessible regions between multiple atac-seq samples to get the reads per region in each sample. My goal is to make a heatmap to visually represent the change in accessibility. As each sample wasn't sequenced to the same depth and the number of mapped reads isn't equal, how can I normalize the FeatureCounts results for each sample?