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  • sublimetech
    Junior Member
    • Nov 2014
    • 9

    BLAST taxid to complete linage using biopython on a local machine.

    Hello all,

    Using BLAST, I am trying to identify thousands of sequences. To make my job a bit easier, I would like to classify these sequences first by sorting them on phylum e.g. only keep BLAST hits from a certain pylum. To do this, I need to generate het complete linage of the hit using the NCBI taxonomic database. I know you can query the database with biopython. over the internet, but the machine I am working on has no internet access.

    Could anyone give me some insight into this?

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  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
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