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  • Izal
    Junior Member
    • Jan 2018
    • 5

    #1

    InParanoid blastall and formatb ERROR

    Hi everyone!

    I'm trying to find protein orthologs between strains using InParanoid.
    But when I execute it, I obtain errors in <formatdb> and <blast-all> phase.
    Is this normal? Or am I doing something wrong? The command seems simple.
    Thanks in advance!!!


    Command:
    $ perl inparanoid.pl strain1.fa strain2.fa


    Here, a part of the Output with errors:
    Trying to run BLAST now - this may take several hours ... or days in worst case!
    Formatting BLAST databases
    Done formatting
    Starting BLAST searches...

    Starting first BLAST pass for strain1.fa - strain1.fa on mar abr 24 20:53:57 CEST 2018
    [blastall] WARNING: the -C 3 argument is currently experimental


    Starting second BLAST pass for strain1.fa - strain1.fa on mar abr 24 20:54:38 CEST 2018
    [formatdb] WARNING: Cannot add sequence number 1 (lcl|1_./tmpd) because it has zero-length.

    [formatdb] FATAL ERROR: Fatal error when adding sequence to BLAST database.
    [blastall] FATAL ERROR: -triphosphate: Database ./tmpd was not found or does not exist

    no element found at line 1, column 0, byte -1 at ./blast_parser.pl line 110.
    [formatdb] WARNING: Cannot add sequence number 2 (lcl|2_./tmpd) because it has zero-length.

    [formatdb] FATAL ERROR: Fatal error when adding sequence to BLAST database.
    [blastall] FATAL ERROR: NZ_000411.1_prot_WP_011112711.1_1: Database ./tmpd was not found or does not exist
  • Izal
    Junior Member
    • Jan 2018
    • 5

    #2
    solved :-)

    Comment

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