Hi,
After IMHO intensive search in the internet for help, I must admit that i couldn't find anything too helpful, so I would like to ask here if there is a reasonable explanation or method to do that.
I am working with mRNA-Seq from Drosophila and after running the fastqc software got a very high duplication level.
I explaind it more specifically here (http://seqanswers.com/forums/showthr...1804#post31804), But as I think that it is an important question (at least for me), I would like to ask it separately again here.
It will be nice to get some data from both sides of the analysis. If someone has data of PCR duplications he/she can give, I would like to have a look at it.
Was it expected to have something like that in a RNA-Seq experiment?
How can someone reliably differentiate between the two cases?
Thanks for the help
Assa
After IMHO intensive search in the internet for help, I must admit that i couldn't find anything too helpful, so I would like to ask here if there is a reasonable explanation or method to do that.
I am working with mRNA-Seq from Drosophila and after running the fastqc software got a very high duplication level.
I explaind it more specifically here (http://seqanswers.com/forums/showthr...1804#post31804), But as I think that it is an important question (at least for me), I would like to ask it separately again here.
It will be nice to get some data from both sides of the analysis. If someone has data of PCR duplications he/she can give, I would like to have a look at it.
Was it expected to have something like that in a RNA-Seq experiment?
How can someone reliably differentiate between the two cases?
Thanks for the help
Assa
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