Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • teaelleceecee
    Junior Member
    • Apr 2015
    • 1

    Program to view multiple alignment files at once

    I have aligned reads to a database containing 10 reference sequences using BWA MEM, and now have .sam and .bam files.

    Is there a program that would allow me to few those 10 alignments within the one .sam/.bam file all at once?

    I am currently using Tablet, but this only allows me to view the alignment for each reference sequence one at a time.

    Alternatively, is there a program that would allow me to view multiple .sam/.bam files at once, when each of those .sam/.bam files have only 1 reference sequence?

    I'm sorry if this question has been answered before but I couldn't find a post that answered this for me.

    Thank you for your help.
  • GenoMax
    Senior Member
    • Feb 2008
    • 7142

    #2
    Are you sure about tablet not being able to open more than one alignment?

    In any case IGV will allow you to open multiple files (aligned to same genome). You will need to make sure you have enough RAM available and change the startup script for IGV to make more RAM available as needed.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      Cancer Drug Resistance: The Lingering Barrier to Rising Survival
      by SEQadmin2



      Cancer survival rates have significantly increased in the last few decades in the United States, reaching a combined 70% 5-year survival rate by 2021. Behind this number, there are years of research to find new therapies, drug targets, and early detection methods. But there is one core challenge that keeps slowing down these advances, and it’s about drug resistance.

      There is no single reason why many patients don’t respond to treatment as expected. Cancer is...
      Today, 05:17 AM
    • GATTACAT
      Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by GATTACAT
      Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
      07-01-2026, 11:43 AM
    • SEQadmin2
      Nine Things a Sample Prep Scientist Thinks About Before Sequencing
      by SEQadmin2


      I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

      Here are nine questions we think about, in roughly the order they matter, before...
      06-18-2026, 07:11 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, Today, 10:08 AM
    0 responses
    6 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, Yesterday, 11:05 AM
    0 responses
    8 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 07-02-2026, 11:08 AM
    0 responses
    31 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 06-30-2026, 05:37 AM
    0 responses
    28 views
    0 reactions
    Last Post SEQadmin2  
    Working...