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RNASeq, Whole Genome & MetaGenomics Training in Dubai

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  • RNASeq, Whole Genome & MetaGenomics Training in Dubai

    2-day Technical Training in NGS Data Analysis | DNA, RNA & MetaGenomics in Dubai
    When: 6-7 April 2020
    Where: Executive Lounge, Business Centre, Level 30, The H Hotel-Office Tower, #1 Sheikh Zayed Road, Dubai, United Arab Emirates
    This will be our 6th event in Dubai

    Event Webpage

    PRACTICAL APPLICATION: In the workshop, we will perform 3 experiments to analyze the example RNASeq, DNASeq & MetaGenomics data. Hands-on exercises will be performed individually using software tools on your own laptops. No prior software experience is required.
    SCHEDULE | DAY 1
    EXPERIMENT 1

    In this lesson, we will focus on the SNPs and Variant Calling (VC) of the DNASeq data and cover:
    • Deal with the raw data for quality evaluation and filtering
    • Map reads to the reference genomes, call SNPs and predict their phenotype consequence
    • Alignment to a reference genome, SNPs and In-dels Calling of next-generation sequencing DNA data


    SCHEDULE | DAY 2
    EXPERIMENT 2

    In this lesson, we will focus on the Differential expression of Genes (DEGs) of the RNASeq data and will cover:
    • Upload Data
    • Convert File Format
    • Align Reads To Reference Genome
    • View The Mapped Reads In JBrowse
    • Count Reads Per Gene
    • Test For Differential Expression
    • Differential Gene Expression
    • MDS Plot
    • MA Plot
    • Volcano Plot
    • Parallel Coordinates and Heatmap
    • Investigate Differentially-Expressed Genes
    • Chord Diagram


    EXPERIMENT 3
    In this lesson, we will focus on the MetaGenomics Analysis in 16S rRNA | Amplicon Sequencing Data Analysis (OTUs) and will cover:
    • File Optimization- Loading of data & optimizing it according to our experiment.
    • Quality Control- Check sequencing quality
    • Sequence Alignment- Grouping/Alignment of reads
    • Extraction of Taxonomic Information- Operational Taxonomic Units (OTUs)- Clustering Sequences
    • Visualization- Examine diversity of species/taxa present within a single sample, Comparison of species/taxa diversity between two samples & Taxonomic Diversity- How many species are in a sample? What are the differences in species between samples?
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