I performed an experiment with a large RNA-seq dataset and I added mRNA stability assay to it. I want to analyze the factors that impact mRNA stability in my assay, and I was wondering if I can analyze codon usage/bias in RNA-seq data without performing Ribo-seq. Is there a script or software that can count and quantify codons in RNA-seq datasets? Any help would be really great.
Thanks!
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The complexity of cancer is clearly demonstrated in the diverse ecosystem of the tumor microenvironment (TME). The TME is made up of numerous cell types and its development begins with the changes that happen during oncogenesis. “Genomic mutations, copy number changes, epigenetic alterations, and alternative gene expression occur to varying degrees within the affected tumor cells,” explained Andrea O’Hara, Ph.D., Strategic Technical Specialist at Azenta. “As...-
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