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  • sweet_dna_girl
    Member
    • Apr 2011
    • 11

    #1

    short read qualities for various NGS machines

    Hi,

    I am looking for the DNA sequence coverage profiles for the human genome for various different sequencing machines, such as Illumina HiSeq, ABI Solid, Pac Biosciences, and Ion Torrent.

    Basically, I would like to see where the gaps (places where there is little to no coverage) are for the different machines in the human genome sequence.

    Also, I am looking for the short read error profiles for these machines. I need to know for the short reads for each machine, where the errors tend to occur.

    Does anyone know where I can find a paper or a website related to this?

    I know this isn't that easy to find, so thanks for your help!!!!
  • sweet_dna_girl
    Member
    • Apr 2011
    • 11

    #2
    hi, does anyone know anything about this??? Please help!

    Comment

    • nilshomer
      Nils Homer
      • Nov 2008
      • 1283

      #3
      There is no substitute for real data. Have you tried downloading some and seeing for yourself? Otherwise you could go through the supplements in the various genome papers (google can find those for you).

      Comment

      • sweet_dna_girl
        Member
        • Apr 2011
        • 11

        #4
        ok thanks! but I will need unfiltered data, not just the good quality short reads, so that I can really see how the machines are performing. do you know where I can find this?

        Comment

        • ECO
          --Site Admin--
          • Oct 2007
          • 1360

          #5
          I see you posted a very similar question again (here: http://seqanswers.com/forums/showthr...?t=11172)...so I'm closing this thread.

          Comment

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