Unconfigured Ad

Collapse
X
 
  • Time
  • Show
Clear All
new posts
  • Azazel
    Member
    • Oct 2010
    • 52

    #1

    Segmentation fault with samtools rmdup

    I am running into a Seg fault when using samtools rmdup.

    I have paired-end alignments from bwa and filter them so that I retain only those pairs which are actually pairs, which have both fragments properly aligned, with no fragment unmapped, and with no next fragment unmapped:

    Code:
    ~/samtools-0.1.12a/samtools view -h -b -F 0xC -f 0x3 input.bam -o ~/foobar.bam
    Now I want to remove duplicates...

    Code:
    ~/samtools-0.1.12a/samtools rmdup ~/foobar.bam ~/rmdupped.bam
    ...and get this:

    Code:
    [bam_rmdup_core] processing reference chr2...
    [...] (long output omitted, here it goes through the chromosomes)
    [bam_rmdup_core] processing reference chr2...
    [bam_rmdup_core] 1 unmatched pairs
    [bam_rmdup_core] processing reference chrX...
    Segmentation fault
    My questions are:

    What can I do about the Segmentation fault?

    Why does it report "1 unmatched pairs", I thought that through the previous filtering (see above) there can not be any unmatched pairs anymore? How can I identify the unmatched pair, if there really is one (I tried samtools view -f 0x4, that doesn't find anything though)?

    I am using samtools version Version: 0.1.12a (r862).

    Any help would be much appreciated. Thanks.
  • deyler
    Member
    • Sep 2013
    • 20

    #2
    perhaps sort first?

    Have you tried sorting first?

    The samtools man page says:

    samtools rmdup [-sS] <input.srt.bam> <out.bam>

    Which implies that the input should be sorted.

    Comment

    Latest Articles

    Collapse

    • SEQadmin2
      16S/ITS Profiling vs. Shotgun Metagenomics: What Changes in Your Workflow?
      by SEQadmin2




      NGS has revolutionized clinical microbiology by offering new ways to identify and surveil pathogens without prior knowledge of the target. One popular approach is 16S rRNA and ITS amplicon sequencing, which targets specific microbial markers. But studies are increasingly incorporating shotgun metagenomics, a technique that sequences all DNA from a sample without targeting specific microbial markers. This approach allows more detailed taxonomic classification, detection...
      Today, 06:46 AM
    • SEQadmin2
      Multiomic Liquid Biopsy: What DNA/RNA Panels Reveal That DNA Alone Can’t
      by SEQadmin2



      Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?

      Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating
      ...
      10-14-2026, 09:55 AM

    ad_right_rmr

    Collapse

    News

    Collapse

    Topics Statistics Last Post
    Started by SEQadmin2, 10-28-2026, 09:57 AM
    0 responses
    23 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-28-2026, 09:51 AM
    0 responses
    15 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 11:13 AM
    0 responses
    68 views
    0 reactions
    Last Post SEQadmin2  
    Started by SEQadmin2, 10-22-2026, 10:48 AM
    0 responses
    48 views
    0 reactions
    Last Post SEQadmin2  
    Working...