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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Started by laura, 05-09-2011, 04:42 AM
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35 responses
107,296 views
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by laura
12-05-2013, 02:37 AM
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Started by sci_guy, 01-23-2008, 11:19 PM
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236 responses
682,581 views
1 reaction
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by ECO
12-25-2009, 06:45 PM
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Started by bmtb, 10-12-2012, 02:52 PM
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59 responses
44,096 views
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by BasesBreaker
08-28-2023, 10:50 AM
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Started by JonasBehr, 08-23-2023, 06:18 AM
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0 responses
126 views
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by JonasBehr
08-23-2023, 06:18 AM
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Started by Arkajyoti Banerjee, 08-22-2023, 12:48 PM
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139 views
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Nanopore reads begin and end in an A polymer region of Arabidopsis but only in one direction?
by dbcrosbie
Started by dbcrosbie, 08-07-2023, 12:50 AM
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1 response
242 views
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by GenomicSeq
08-11-2023, 09:02 AM
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Started by ehaag, 08-08-2023, 01:09 PM
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1 response
71 views
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by Ben3
08-08-2023, 02:01 PM
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Started by lre1234, 07-26-2023, 11:58 AM
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1 response
77 views
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by GenomicSeq
07-28-2023, 11:22 AM
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Started by ecSeq Bioinformatics, 05-07-2023, 11:46 PM
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4 responses
192 views
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Started by Perseo, 07-19-2023, 06:32 AM
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3 responses
798 views
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by GenomicSeq
07-26-2023, 05:13 AM
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Started by Sapuizait, 07-22-2023, 02:53 AM
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0 responses
238 views
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by Sapuizait
07-22-2023, 02:53 AM
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Started by crazyhottommy, 07-21-2023, 04:36 AM
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0 responses
96 views
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by SEQadmin2
Childhood cancers, effectively fatal 60 years ago, are now curable for up to 85% of children with access to contemporary treatments and...-
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05-08-2026, 01:42 AM -
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Channel: Articles
Yesterday, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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by SEQadmin2
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Channel: Articles
06-02-2026, 10:05 AM -
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Started by SEQadmin2, Today, 11:08 AM
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6 views
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by SEQadmin2
Today, 11:08 AM
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Started by SEQadmin2, 06-30-2026, 05:37 AM
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0 responses
11 views
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by SEQadmin2
06-30-2026, 05:37 AM
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Started by SEQadmin2, 06-26-2026, 11:10 AM
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0 responses
19 views
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by SEQadmin2
06-26-2026, 11:10 AM
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