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Discussion of next-gen sequencing related bioinformatics: resources, algorithms, open source efforts, etc
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Started by laura, 05-09-2011, 04:42 AM
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35 responses
107,288 views
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by laura
12-05-2013, 02:37 AM
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Started by sci_guy, 01-23-2008, 11:19 PM
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236 responses
682,472 views
1 reaction
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by ECO
12-25-2009, 06:45 PM
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Started by jazz710, 03-04-2016, 09:40 AM
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4 responses
4,310 views
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by jazz710
03-14-2016, 08:08 AM
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Started by Jane M, 03-11-2016, 07:46 AM
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6 responses
3,153 views
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by Jane M
03-14-2016, 07:33 AM
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Started by Jessica_L, 03-14-2016, 07:20 AM
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0 responses
1,777 views
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by Jessica_L
03-14-2016, 07:20 AM
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Started by sequence_hard, 03-14-2016, 05:48 AM
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1 response
2,188 views
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by GenoMax
03-14-2016, 05:59 AM
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Started by simon_seq, 03-14-2016, 04:42 AM
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1 response
2,616 views
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by GenoMax
03-14-2016, 04:50 AM
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Started by standonn, 03-11-2016, 02:36 AM
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4 responses
3,224 views
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by standonn
03-14-2016, 04:34 AM
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Started by Luis, 03-12-2016, 03:15 AM
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3 responses
4,361 views
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by yueluo
03-13-2016, 08:31 PM
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Started by Bara'a Al-Hammad, 03-09-2016, 01:28 PM
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2 responses
1,623 views
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Started by dr_bantz, 03-12-2016, 04:02 PM
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0 responses
1,316 views
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by dr_bantz
03-12-2016, 04:02 PM
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Started by Jen99, 03-12-2016, 02:48 PM
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by SEQadmin2
Childhood cancers, effectively fatal 60 years ago, are now curable for up to 85% of children with access to contemporary treatments and...-
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05-08-2026, 01:42 AM -
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Channel: Articles
Today, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
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by SEQadmin2
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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Channel: Articles
06-02-2026, 10:05 AM -
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Started by SEQadmin2, Yesterday, 05:37 AM
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8 views
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by SEQadmin2
Yesterday, 05:37 AM
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Started by SEQadmin2, 06-26-2026, 11:10 AM
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0 responses
17 views
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by SEQadmin2
06-26-2026, 11:10 AM
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Whole-Genome Sequencing Traces Faroe Islands Ancestry to a North Atlantic Founder Population
by SEQadmin2
Started by SEQadmin2, 06-17-2026, 06:09 AM
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0 responses
52 views
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by SEQadmin2
06-17-2026, 06:09 AM
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