Pacbio - What is the Typical Throughput?

[samanta]

Based on sparsity of pacbio-related posts, I presume nobody uses the technology. I was at their recent user group meeting and liked the smell of the coffee

http://www.homolog.us/blogs/blog/2013/09/21/end-illumina-era/

http://www.homolog.us/blogs/blog/2013/09/22/end-short-read-era-part-ii/

One question, where I presume the company's estimates could be on the high side is the throughput per SMRT cell. What is your usual experience? Are the numbers listed here achievable?

The last number from krobison (81Mb) is quite low compared to what I heard at the meeting (400-500 Mb). Also mention the chemistry you are using.

[GenoMax]

With XL chemistry we have recently typically seen 240-270 Mb per smrtcell with 5-6 kb reads (1 x 120 min on an RSII machine).

[flxlex]

Before P4, 150-300 was common. Our best P4-C2 run was a raw read average length of 7.6 kbp and yielded 730 Mbp (!) for a CCS project (96k reads, but the insert was only 500 bp).

[samanta]

Thanks folks.

Flxlex, I presume that 730 Mb is one time observation, right?

[flxlex]

Thanks folks.

Flxlex, I presume that 730 Mb is one time observation, right?

The run was an outlier, yes, with the 730 being the best SMRTcell...

[chjp0632]

I am curious the cost for one SMRT cell sequencing (not including human force) ?

[GenoMax]

I am curious the cost for one SMRT cell sequencing (not including human force) ?

Here is a list of the PacBio sequence providers. http://www.pacificbiosciences.com/su...cing_provider/ You will have to ask for a quote.