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Alternative to Qiagen Puregene Tissue kit for HMW gDNA?

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  • Alternative to Qiagen Puregene Tissue kit for HMW gDNA?

    Hello,

    We're extracting high molecular weight gDNA for PacBio from an insect-a bee- using the Qiagen Puregene Tissue kit (4g). We have trouble with this kit back-ordered all the time. Could someone from the community recommend an alternative for HMW gDNA that works well on tissue for PacBio?

    Thanks,

    Dacotah

  • #2
    I have tried the following protocol with insects and it works quite well in terms of purity and length:

    Modified low‐salt CTAB extraction of high‐quality DNA from contaminant‐rich tissues

    https://onlinelibrary.wiley.com/doi/...755-0998.12616

    A benchtop protocol is available in the "Supporting Information" section.

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    • #3
      Hi Dacotah,

      Qiagen's PureGene kits are nearly identical to the former Epicentre MasterPue DNA Purification kits, which are now available through Lucigen/Biosearch Technologies. Catalog numbers would be MC85200 for 200 reactions and MC89010 for 10 reactions. Both are non-column based products.

      Olafblue1955

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      • #4
        Oh, that's great, thank you very much.

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