I am trying to use fastX barcode splitter perl script on my fastq file. However, every time I run the program I get this message Error: barcode file not specified (use '--bcfile [FILENAME]'). All I am doing is dragging the perl script into terminal, and dragging the fastq file into the terminal and hit enter and that what i get. Can anybody please help me
-
Last edited by mjose23; 10-16-2013, 01:35 PM. -
Drag and drop is probably not going to work. Instead open the terminal and then on the command line prompt run the program (you will have to take care of all file paths) following the usage example shown here: http://hannonlab.cshl.edu/fastx_tool...splitter_usageOriginally posted by mjose23 View PostLast edited by GenoMax; 10-16-2013, 02:27 PM. -
To expound a bit on GenoMax's reply, you're only dragging and dropping part of the full command (the error message you received indicates this). In addition to the perl script and the fastq file, you need to type " --bcfile " and then drag the file specifying the barcodes after that. Reading error messages will usually allow you to figure out what's wrong.Comment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment