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I am trying to use fastX barcode splitter perl script on my fastq file. However, every time I run the program I get this message Error: barcode file not specified (use '--bcfile [FILENAME]'). All I am doing is dragging the perl script into terminal, and dragging the fastq file into the terminal and hit enter and that what i get. Can anybody please help me
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Last edited by mjose23; 10-16-2013, 01:35 PM. -
Drag and drop is probably not going to work. Instead open the terminal and then on the command line prompt run the program (you will have to take care of all file paths) following the usage example shown here: http://hannonlab.cshl.edu/fastx_tool...splitter_usageOriginally posted by mjose23 View PostLast edited by GenoMax; 10-16-2013, 02:27 PM. -
To expound a bit on GenoMax's reply, you're only dragging and dropping part of the full command (the error message you received indicates this). In addition to the perl script and the fastq file, you need to type " --bcfile " and then drag the file specifying the barcodes after that. Reading error messages will usually allow you to figure out what's wrong.Comment
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