I am trying to use fastX barcode splitter perl script on my fastq file. However, every time I run the program I get this message Error: barcode file not specified (use '--bcfile [FILENAME]'). All I am doing is dragging the perl script into terminal, and dragging the fastq file into the terminal and hit enter and that what i get. Can anybody please help me
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Originally posted by mjose23 View PostI am trying to use fastX barcode splitter perl script on my fastq file. However, every time I run the program I get this message Error: barcode file not specified (use '--bcfile [FILENAME]'). All I am doing is dragging the perl script into terminal, and dragging the fastq file into the terminal and hit enter and that what i get. Can anybody please help meLast edited by GenoMax; 10-16-2013, 02:27 PM.
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To expound a bit on GenoMax's reply, you're only dragging and dropping part of the full command (the error message you received indicates this). In addition to the perl script and the fastq file, you need to type " --bcfile " and then drag the file specifying the barcodes after that. Reading error messages will usually allow you to figure out what's wrong.
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