Unconfigured Ad

Collapse
X
 
  • Filter
  • Time
  • Show
Clear All
new posts
  • Carcharodon
    Member
    • Jul 2015
    • 40

    Greetings from Honolulu, HI!

    Hello, all!

    I'm afraid I've jumped the gun and have already made a thread asking for help, but now is as good a time as any to make an introduction.

    I am a PhD candidate at the University of Hawaii at Manoa entering his fourth year in the Zoology program.

    Briefly, I am interested in the effects of life history - particularly dispersal capability - on the evolution/diversity and population structure of elasmobranch fishes (sharks, skates, and rays). To that end, my dissertation will focus on evolutionary patterns and processes in deep evolutionary time (phylogenetic methods), phylogeography, and population structure (population genetics).

    I'm currently attempting to examine the population structure of a shark with exceptionally limited dispersal capability using next-generation sequencing methods. In particular, I'm hoping to employ ddRAD in order to generate a large number of SNPs which may be useful for population genetic analyses. I'm currently hoping to use the Illumina MiSeq platform, but may decide to move to the Illumina HiSeq platform as my collection of samples and multiplexing needs continue to grow.

    I've been lurking for some time, and this seems like an awesome community. I look forward to my time here with all of you!

    Pleased to make your acquaintance,

    Sean

Latest Articles

Collapse

  • GATTACAT
    Reply to Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by GATTACAT
    Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
    07-01-2026, 11:43 AM
  • SEQadmin2
    Nine Things a Sample Prep Scientist Thinks About Before Sequencing
    by SEQadmin2


    I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.

    Here are nine questions we think about, in roughly the order they matter, before...
    06-18-2026, 07:11 AM

ad_right_rmr

Collapse

News

Collapse

Topics Statistics Last Post
Started by SEQadmin2, 07-02-2026, 11:08 AM
0 responses
18 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-30-2026, 05:37 AM
0 responses
19 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-26-2026, 11:10 AM
0 responses
21 views
0 reactions
Last Post SEQadmin2  
Started by SEQadmin2, 06-17-2026, 06:09 AM
0 responses
54 views
0 reactions
Last Post SEQadmin2  
Working...