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Started by ECO, 11-21-2011, 08:25 PM
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by plant_hunt
11-06-2019, 05:43 AM
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Started by wyeehlrit, Today, 11:50 AM
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by wyeehlrit
Today, 11:50 AM
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Started by wyeehlrit, Today, 07:57 AM
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by wyeehlrit
Today, 07:57 AM
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Started by JeefreyH, Today, 06:05 AM
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by JeefreyH
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Started by wyeehlrit, Today, 06:01 AM
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Started by Javislola, Today, 04:04 AM
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by Javislola
Today, 04:04 AM
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Started by Sunflower34, 06-03-2026, 05:05 AM
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by Sunflower34
06-03-2026, 05:05 AM
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Started by SouBen, 06-02-2026, 02:43 AM
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by SouBen
06-02-2026, 02:43 AM
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Started by sophiasmith, 04-28-2026, 01:00 AM
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by sophiasmith
04-28-2026, 01:00 AM
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Started by ysevel, 04-20-2026, 11:42 PM
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by SEQadmin2
04-22-2026, 09:22 AM
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Started by JW_REGN, 03-24-2026, 07:49 AM
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by JW_REGN
03-24-2026, 07:49 AM
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by GATTACATLove this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.
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Channel: Articles
07-01-2026, 11:43 AM -
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by SEQadmin2
I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...-
Channel: Articles
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by SEQadmin2
Data variability is still an issue in sequencing technologies despite the advances in reproducibility and accuracy of these platforms. But the problem does not originate in the sequencing itself, but in the previous steps, before the sample reaches the sequencer.
The first step is collection, followed by preservation and sample preparation for analysis. Most scientists overlook those steps, but not being careful might just be skewing the experiment’s results.
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06-02-2026, 10:05 AM -
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Started by SEQadmin2, Today, 11:05 AM
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Today, 11:05 AM
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Started by SEQadmin2, 07-02-2026, 11:08 AM
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07-02-2026, 11:08 AM
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Started by SEQadmin2, 06-30-2026, 05:37 AM
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by SEQadmin2
06-30-2026, 05:37 AM
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