Tweet
Hi Everyone,
As per my last post I would like to start organising a one day workshop for sample-prep issues. This is most likely going to focus on Illumina sequencing but I am happy to be platform agnostic where appropriate and have open discussions.
Watch this space for more developments.
I would like to ask you to think about the impact and relevance of a one day meeting focussing on sample prep and applications of next-gen sequencing. This would be a similar format to the Bioinformatics meeting yesterday. I have listed some possible topics below and would appreciate your feedback on this. I would envisage a 5-10 minute presentation from someone with lots of experience giving their recommendations on each topic. This would be followed by a moderated open floor discussion hopefully resulting in a consensus of how best to approach the question. If we go ahead I will ask for volunteers to form an organising committee to help plan the day. I would aim for early September so if you know of conflicting conference or meetings around then please let me know.
Topics for discussion/presentation:
Library quality control and quantification.
SE vs PE library prep.
Sample input requirements.
Illumina vs DIY sample prep.
Multiplexing Genome partitioning: microarray hyb
selection, lrPCR, MIP, RainDance, Agilent OLS, etc.
Applications
RNA-seq: oligodT vs Ribominus, stranded or not, SE vs PE, size
selection, etc. Structural variation: what insert size(s) to use?
ChIP-seq: similar questions to above but relevant to ChIP experiments.
SmallRNA-seq: similar questions to above but relevant to SmallRNA experiments.
Would you be interested in attending or even leading a session? Would a one day meeting be too much or too little? How 'open' would you want this meeting to be; our email list, Cambridge or UK wide. Would you prefer a focus on Illumina technology or discussion on some of the competition?
Sincerely,
James.
As per my last post I would like to start organising a one day workshop for sample-prep issues. This is most likely going to focus on Illumina sequencing but I am happy to be platform agnostic where appropriate and have open discussions.
Watch this space for more developments.
I would like to ask you to think about the impact and relevance of a one day meeting focussing on sample prep and applications of next-gen sequencing. This would be a similar format to the Bioinformatics meeting yesterday. I have listed some possible topics below and would appreciate your feedback on this. I would envisage a 5-10 minute presentation from someone with lots of experience giving their recommendations on each topic. This would be followed by a moderated open floor discussion hopefully resulting in a consensus of how best to approach the question. If we go ahead I will ask for volunteers to form an organising committee to help plan the day. I would aim for early September so if you know of conflicting conference or meetings around then please let me know.
Topics for discussion/presentation:
Library quality control and quantification.
SE vs PE library prep.
Sample input requirements.
Illumina vs DIY sample prep.
Multiplexing Genome partitioning: microarray hyb
selection, lrPCR, MIP, RainDance, Agilent OLS, etc.
Applications
RNA-seq: oligodT vs Ribominus, stranded or not, SE vs PE, size
selection, etc. Structural variation: what insert size(s) to use?
ChIP-seq: similar questions to above but relevant to ChIP experiments.
SmallRNA-seq: similar questions to above but relevant to SmallRNA experiments.
Would you be interested in attending or even leading a session? Would a one day meeting be too much or too little? How 'open' would you want this meeting to be; our email list, Cambridge or UK wide. Would you prefer a focus on Illumina technology or discussion on some of the competition?
Sincerely,
James.
Comment