Low Enrichments using Lib A EMPCR kits with SV emulsions
We are seeing similar kind of problems with our emPCR. The enrichment are very low even after increasing the CPB from 1CPB to 8 CPB! The enrichments still remains under 3%. We quantify our libraries using the Kapa QPCR kits which has been working very well previously and we used 1CPB or max 2CPB to get perfect enrichment. But for the last three FLX runs, the enrichment is considerably low even at higher cpb and the readlength have dropped from average 400bp to 250bp.
Still debating if it is the libraries, thermocycler or the emPCR kit.
We are seeing similar kind of problems with our emPCR. The enrichment are very low even after increasing the CPB from 1CPB to 8 CPB! The enrichments still remains under 3%. We quantify our libraries using the Kapa QPCR kits which has been working very well previously and we used 1CPB or max 2CPB to get perfect enrichment. But for the last three FLX runs, the enrichment is considerably low even at higher cpb and the readlength have dropped from average 400bp to 250bp.
Still debating if it is the libraries, thermocycler or the emPCR kit.
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