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Hello,
This might be a stupid question.
We are currently working on a ~5M bacterial genome assembly. Our reads data is ~8X coverage, 454 sequencing. The coverage is kind of low. So we used re-guided assembly. Now we found there were still lots of gaps (~500).
If we want to fill in these gaps, should we resequencing the whole genome on 454? It still costs us too much. Does anybody have any other suggestions?
Thank you very much.
Salmon
This might be a stupid question.
We are currently working on a ~5M bacterial genome assembly. Our reads data is ~8X coverage, 454 sequencing. The coverage is kind of low. So we used re-guided assembly. Now we found there were still lots of gaps (~500).
If we want to fill in these gaps, should we resequencing the whole genome on 454? It still costs us too much. Does anybody have any other suggestions?
Thank you very much.
Salmon
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