Short reads in Titanium

[jpp]

Dear all,
We have done our first Titanium run and we have obtained an aceptable number of reads but the length of these reads has been very short in average, 254nt. The library fullfils the specifications and we can not explain the reason for the short reads. Have any of you experienced this problem? Some advice?

Tnak you in advance
JPP

[westerman]

Not in our titanium runs. Although the numbers do bounce around. Our last 4 runs have been 334, 388, 333, and 375 average sequence length. Your 254nt length would be disappointing but I am afraid I don't have any advice except to go on to another experiment and prep and see if you can do better. If not then look and see if there is system-wide problem.