Alternative sequence enrichment methods

[njae4]

I am looking into alternatives to NimbleGen sequence capture prior to Roche/454 GS FLX sequencing. Does anybody have experience of using the following sequence enrichment methods? Can they be used successfully for Roche/454 GS FLX sequencing?

Raindance

http://www.genomeweb.com/sequencing/raindance-debuts-pcr-based-sequence-enrichment-platform

Agilent SureSelect (possibly Illumina only)

http://www.agilent.com/about/newsroom/presrel/2009/05feb-ca09006.html

Any other alternatives?

[dottomarco]

We are going to test Combimatrix's arrays in the following weeks.
Have you ever tryed any of the methods you mentioned before? How did they work?

[bioinfosm]

I checked Combimatrix website, but am not sure how their exon capture works. Could you share some detail?

[dottomarco]

They do not have a sequence enrichment product (array) out yet. What we are trying to do is testing their arrays to see if they could be usable (and most interestingly for $ , REUSABLE) for seq enrichment. The method of hibridization would be the same as with Nimblegen's or Agilent's arrays.
Unfortunately the samples are not back yet.. so I ll keep you updated when we have some results from the sequencer