GS FLX & Illumina

[FLXTech]

Hi guys,

I'm new to nextgen sequencing technologies. We have started using 454/FLX technology. Can anyone tell me how Illumina differs from FLX? Is it possible to sequence samples enriched on Agilent (sureselect) by FLX.
Any thoughts appreciated.

[swbarnes2]

Illumina and solid both take DNA fragments, and rather than giving you a few hundred bases, like 454 does, it gives you about 50 bases of each end, and it gives you a whole lot more of them. 10-20 million per lane, 8 lanes a run. If you have enough fragments, then having an idea of the distance between your two ends is almost as good as having the whole sequence.

I don't see why Agilent wouldn't work with 454, but Roche owns Nimblegen, so they will steer you towards that technology, and not Agilent's. And that means that Agilent has a lot more incentive to work with Solid and Illumina, so you might not get as much support from Agilent as illumina users would.

[FLXTech]

Illumina

Thanks for the reply, swbarnes2. I'm interested to know more about Illumina's work flow. Is it available on the web?

[joa_ds]

http://www.illumina.com will help you.

[FLXTech]

Thanks, joa_ds