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Is anyone else using both a Junior and a FLX system? Have you noticed a difference in the cpb when moving from one kit to the other? It looks like we need to lower the cpb when moving from the FLX to the Junior, but we woud like confirmation that others are seeing that too.
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Haven't used a Jr kit, but I do notice inconsistencies between the various sized emPCR kits for the FLX, so it's probably a similar phenomenon. I usually decrease the cpb slightly when going from the SV titration to the MV or LV bulk rxns. It doesn't always work out this way though. -
hi Suefo,
I've used both. We used 2cbp for Junior and 3cpb for FLX (or ligated library in both cases) and it seemed to work well.Comment
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Hi, does everyone use the emPCR Lib-A MV kit for GS Junior run? (we can perform the run 4 times). And how long does the kit last since the date it opened? Thank you!Comment
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Love this - good data definitely starts from good input, and poor input can only give relatively poor data. I particularly like the mention of Nanodrop/absorbance based methods for quantification. It's such a toss up if you'll get an accurate reading or what amounts to a randomly generated number, and a lot of library/sequencing related issues can be traced back to poor quant.07-01-2026, 11:43 AM
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I’m not a sequencing expert. I’m a purification scientist who uses NGS to evaluate workflows my group develops. With this perspective, we think about the sample first and the NGS workflow second. The sequencer is an exceptionally honest reporter, but it can only report on what you give it, so whether you get clean, interpretable data from an NGS workflow is largely determined before you begin.
Here are nine questions we think about, in roughly the order they matter, before...
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