Hi 454 users,
I've never used the emPCR kit for amplicon libraries before and get confused about the terminology of A/B for capture beads, amplification primers, enrichment primers and sequencing primers.
My hunch is capture beads A should go with amplification primer A, enrichment primer A and sequencing primer A (and same for B) but I would like to confirm with you before I add wrong primers to the capture beads...
Thanks in advance!
I've never used the emPCR kit for amplicon libraries before and get confused about the terminology of A/B for capture beads, amplification primers, enrichment primers and sequencing primers.
My hunch is capture beads A should go with amplification primer A, enrichment primer A and sequencing primer A (and same for B) but I would like to confirm with you before I add wrong primers to the capture beads...
Thanks in advance!
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