Missing sample key pass wells....

[bacterioskeptic]

Hello all,
Have just run a 16S amplicon library on 454 Titanium. The enrichment, granted, was high (above 20%) but from 2E+6 raw wells only 36 sample key pass wells were identified with 0 control passes! The library worked perfectly before. My first assumption (as we are very sure we did nothing stupid) is reagents or kit - but how likely is this? Has anyone experienced something similar before or recently?
Many thanks
B

[ajthomas]

I haven't had this happen to me, but it sure seems to me there was a problem with your sequencing kit or instrument. Even if the library and your beads were no good, the control beads should have sequenced fine. Contact GS Support. They'll have you send them some data from your run and from that they may be able to determine what went wrong. You should probably do this before you do another run in case it's a problem with the instrument. If it's a problem with the kit or the instrument (and you have a service contract) they will probably replace the items lost by the failed run.