Lonza Flash Gel

[GraemeFox]

Hi,

Is anybody using the Lonza Flash Gel system during their 454 sequencing setup?

http://www.lonza.com/group/en/produc.../flashgel.html

It is recommended by Roche in the Rapid Lib. Prep Manual alongside the Agilent Bioanalyzer however given that the Flash Gel is a fraction of the price we are looking at going down that route.

Had a brief demo earlier this week and all looked well but I was just wondering if anyone was already using this bit of kit and had any feedback or advice?

[proteasome]

We use both Lonza Flash Gels and the Bioanalyzer. For established assays we can usually get away with just using a flash gel, however if you're doing shotgun sequencing the bioanalyzer is important for accurate sizing of your library. If you have any nearby labs with a bioanalyzser perhaps you could borrow theirs when you start out, and then switch to just flash gels once you know your technique is good.