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  • sdstella
    Junior Member
    • May 2009
    • 3
    #1

    454 Vs Sanger

    Hey Everyone,

    i am a new member of this amazing forum, and be honest it helps me a lot! i would like to ask if someone can give me main points about 454 Vs Sanger!

    Thanks,

    Tags: None
    • westerman
      Rick Westerman
      • Jun 2008
      • 1104
      #2
      The 454 compared to Sanger generates many more but shorter reads at a much reduced cost-per-base -- at least 1/10th the cost. There is no cloning bias for the 454. There are problems with long homo-polymer runs.

      That is it in a nutshell.

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