Tweet
I guess the title says it all - has anyone tried sequencing amplicons on an FLX+ yet? We're looking at trying it in the next month or two and I was wondering whether anyone else had tried it already and had any success.
-
-
well, I am waiting for the upgrade, I am so doing it. but the is a bump, ROCHE won't support that very run, as amplicons is not listed as FLX+ application, so in case that something went wrong, there will be no replacement or any sort of support. I guess you can do it anyway. tell me what your results will look like.
Thanks
Mo -
We have done amplicons on the FLX plus with mixed results. Sequencing works very well on the amplicons containing only one type of amplicon (median readlengths of 600bps even though seem like there are more sequecing errors). However, if you pool amplicons of different size and different loci, it completely stuffs up (Lot more key pass and filter pass failures). Apparently due to high signal intensities. Since, Roche will not support it hard to get much information out from them. Not sure why this happens but would be great to get some feedback from any one who has been able to get it working.Comment
-
We've seen the same problem: when mixing amplicons, particularly of different sizes, FLX+ has significant issues. I would note that we routinely pool different sized amplicons on a GS-Jr and don't see any abnormalities in read length or distribution. In the FLX+ runs we've done the reads are either short or scant or bothComment
-
Cancer research has been transformed through numerous molecular techniques, with RNA sequencing (RNA-seq) playing a crucial role in understanding the complexity of the disease. Maša Ivin, Ph.D., Scientific Writer at Lexogen, and Yvonne Goepel Ph.D., Product Manager at Lexogen, remarked that “The high-throughput nature of RNA-seq allows for rapid profiling and deep exploration of the transcriptome.” They emphasized its indispensable role in cancer research, aiding in biomarker...09-07-2023, 11:15 PM -
Ribonucleic acid (RNA) represents a range of diverse molecules that play a crucial role in many cellular processes. From serving as a protein template to regulating genes, the complex processes involving RNA make it a focal point of study for many scientists. This article will spotlight various methods scientists have developed to investigate different RNA subtypes and the broader transcriptome.
Whole Transcriptome RNA-seq
Whole transcriptome sequencing...08-31-2023, 11:07 AM
Comment