Hi,
Our local sequencing provider says the soon-to-be-released Titanium PE kits produce a mix (about half-half) of shotgun reads and PE reads on a given run, where the shotgun reads come from the non-adapted ("internal") part of the circularised molecule. The only info I've seen on 454 Titanium PE sequencing is this ad from Roche: http://www.454.com/downloads/DeNovoC...omes_Flyer.pdf
Thanks!
Anar
Our local sequencing provider says the soon-to-be-released Titanium PE kits produce a mix (about half-half) of shotgun reads and PE reads on a given run, where the shotgun reads come from the non-adapted ("internal") part of the circularised molecule. The only info I've seen on 454 Titanium PE sequencing is this ad from Roche: http://www.454.com/downloads/DeNovoC...omes_Flyer.pdf
- Does anyone know how many shotgun and/or PE reads one expects to get from a single plate of Titanium PE sequencing?
- Can anyone clarify whether there is a selection step for PE constructs prior to sequencing - if so why are non-PE shotgun sequences still prevalent?
Thanks!
Anar
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