Picogreen vs Kappa quantitation kits

[sanju0891]

Hello folks,

According to the threads, everybody suggests Kappa Quantification kit than Picogreen which is recommended by Roche.

Why is it so? and what is the difference between them in results? can someone explain me indetail.

can the picogreen be used with the Lightcycler 480?

Thanks

[Anthony.287]

For me, it depends on what I am doing. For pooling a large number of amplicons, the PicoGreen is much easier for me. Once they are pooled, I use the Kapa kit to get a more accurate measurement of the number of molecules that will amplify in emPCR. For Rapid Libraries, I just follow the protocol (not that many customers want rapid libraries...)

The main difference between the methods is that the PicoGreen will measure all of the DNA in the sample, whereas the Kapa kit will only measure the DNA that has the proper sequences and should amplify in emPCR. I've been using the Kapa kit for about 8 months now, and the sequencing results have been steadily improving.