Dear all,
I have the following question:
when working with 454 and more specific the emulsion PCR, it is important that you only have 1 template DNA for each bead, right?
Can someone explain to me then why in the following picture (article from nature, http://www.nature.com/nrg/journal/v1...rg2626_F1.html, first part of the picture or see the attachment) you see 2 template DNA strands in the box with emulsion PCR. THis does not seem logical.
I know its possible to have 2 strands, but then something went wrong with the preperation of the emulsion PCR, I doubt they are showing this here.
thanks in advance
I have the following question:
when working with 454 and more specific the emulsion PCR, it is important that you only have 1 template DNA for each bead, right?
Can someone explain to me then why in the following picture (article from nature, http://www.nature.com/nrg/journal/v1...rg2626_F1.html, first part of the picture or see the attachment) you see 2 template DNA strands in the box with emulsion PCR. THis does not seem logical.
I know its possible to have 2 strands, but then something went wrong with the preperation of the emulsion PCR, I doubt they are showing this here.
thanks in advance
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