Hi, I am new to this forum. I have been sequencing for ~5 months with quite good results. I am wondering if anyone has sequenced beads outside the recommended range by Roche, i.e. 5-20% enrichment? Would it be "safe" to sequence 30% enriched beads, or what can happen? Regards Eva
-
-
I did 33% and it went ok, the mixed beads was a little bit high, but below 20% of the overall reads.
Good luck
-
Thanks, it doesn't sound too bad. If I decide to try I will post the results. Regards/EvaOriginally posted by mohdsos View PostI did 33% and it went ok, the mixed beads was a little bit high, but below 20% of the overall reads.
Good luckComment
-
Tumors contain complex mixtures of cell populations that evolve in response to treatment and host-specific factors. Tissue biopsies provide only static snapshots of a tumor’s state. But what if we could follow that evolution over time using a minimally invasive technique?
Liquid biopsies can detect tumor-derived material circulating in the bloodstream, providing a way to study treatment response, detect emerging resistance, and discover new biomarkers. Circulating...10-14-2026, 09:55 AM -
Researchers using sequencing and genomics tools often have to make trade-offs. They can choose between speed or scale, short reads or long-range information, or targeted panels or a view of the whole transcriptome. New technologies that have been released this year are built to address those tough choices.
We asked six companies the same four questions to learn about their latest products. The new technologies bring a lot to the table, including rethinking sequencing...
Comment