Our customer submitted randomly amplified DNA. Customer said that the dsDNA was synthesized from RNA and DNA. The size of sample is from 100bp over 2.5 kb. I prepared a libray using rapid library preparation method. The libray size was bigger than a library which I prepared using genomic DNA. When I did emPCR but I can't get enough sequencing beads even at 64 cpb. I also try to do emPCR at 4, 12, 24 cpb. I can't get enough sequencing bead. When I did emPCR at 4cpb or 6cpb using a libray of genomic DNA, I got enough sequencing bead.
Do you have any idea about the libray prep. and emPCR? Or does anybody have sequencing experience using randomly amplified DNA?
Hong
Do you have any idea about the libray prep. and emPCR? Or does anybody have sequencing experience using randomly amplified DNA?
Hong
Comment