We recently had six whole genome amplified emPCR's fail. These were from environmental samples. I'm wondering if contaminants have carried through the rapid library prep and killed the reaction. Anybody have similar problems?
Also, what does it mean if control beads are having 60% of their sequences trimmed by the too-short-quality filter? Seems high to me. Only one set of the two control bead types are doing this.
Thanks.
Barry
Also, what does it mean if control beads are having 60% of their sequences trimmed by the too-short-quality filter? Seems high to me. Only one set of the two control bead types are doing this.
Thanks.
Barry
Comment