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  • Anyone have enrichment problems?

    Hello, I have very difficult time with enrichment. Most libraries need high copies per bead to give >5% enrichment rate. I use 10 copies or more. This is strange to me. I usually use much less than 10 copies per bead. I am afraid this will cause mixed Signals. How come I cant get good values anymore? Anyone have this problem? Thank you for help.

  • #2
    Yeah, this seems to be happening for a lot of users. I've ad to go all the way to 32 cpb to get 8% with an amplicon pool. No idea why.

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