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  • chrislove
    Junior Member
    • Aug 2012
    • 1
    #1

    some question in processing 454 data (Pyrobayes, indel)

    Hi,experts!
    Straight to the point！
    How to call indels with 454 data?? I used pyrobayes to re-call base. I got 2 files, a fasta containg sequences and a corresponding fastq, but didn't know how to continue then. How can I get one fastq file from the two? So, I could mapping this fastq file to my reference and get .BAM file for anaysis.
    But I use GATK to call SNP which doesn't call indels in 454 data. Is there a well accepted software can deal with 454 indel?
    Hope and appreciate for your detail help!!
    Chris!
    Tags: None
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