Difference between 454 pair end library and 454 standard library

[edge]

Hi,
Can anybody list out the main difference between the 454 pair end library and 454 standard library?
Thanks a lot

[maubp]

In terms of the SFF files themselves (as far as I know) there is no difference at all. There is nothing in the file format or the Roche XML meta data block that I have seen to tell you if the data is single reads or paired ends.

It is all down to the sample preparation. The Roche 454 website has a nice summary of how this works:

http://www.454.com/products-solutions/experimental-design-options/multi-span-paired-end-reads.asp

If you know you have a paired end SFF file, you should be able to find the linker sequences in the reads - see:

http://seqanswers.com/forums/showthread.php?t=2698