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I got two 16s rRNA data set back from sequence center for a sample for metagenomics project. one set from the FLXA side (> 200 bp) and another set from FLXB side (> 200 bp)
We only have one primer set(F/R).
Because I couldn't understand the process how did they do the bidirection sequencing, I asked them.
They said they have retrofitted and barcoded the selected amplicon samples with
Titanium adaptors and sequenced the whole pool. The retro-fit approach for amplicons implies they can sequence from both sides. They pool the retro-fitted reactions (AA-BB, AB-AB) together in a region.
Could someone explain what does this mean for me?
We only have one primer set(F/R).
Because I couldn't understand the process how did they do the bidirection sequencing, I asked them.
They said they have retrofitted and barcoded the selected amplicon samples with
Titanium adaptors and sequenced the whole pool. The retro-fit approach for amplicons implies they can sequence from both sides. They pool the retro-fitted reactions (AA-BB, AB-AB) together in a region.
Could someone explain what does this mean for me?