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  • plumb
    started a topic what's the retro-fitted reaction in pyrosequencing

    what's the retro-fitted reaction in pyrosequencing

    I got two 16s rRNA data set back from sequence center for a sample for metagenomics project. one set from the FLXA side (> 200 bp) and another set from FLXB side (> 200 bp)

    We only have one primer set(F/R).

    Because I couldn't understand the process how did they do the bidirection sequencing, I asked them.

    They said they have retrofitted and barcoded the selected amplicon samples with
    Titanium adaptors and sequenced the whole pool. The retro-fit approach for amplicons implies they can sequence from both sides. They pool the retro-fitted reactions (AA-BB, AB-AB) together in a region.

    Could someone explain what does this mean for me?

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