I got two 16s rRNA data set back from sequence center for a sample for metagenomics project. one set from the FLXA side (> 200 bp) and another set from FLXB side (> 200 bp)
We only have one primer set(F/R).
Because I couldn't understand the process how did they do the bidirection sequencing, I asked them.
They said they have retrofitted and barcoded the selected amplicon samples with
Titanium adaptors and sequenced the whole pool. The retro-fit approach for amplicons implies they can sequence from both sides. They pool the retro-fitted reactions (AA-BB, AB-AB) together in a region.
Could someone explain what does this mean for me?
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Metagenomics has improved the way researchers study microorganisms across diverse environments. Historically, studying microorganisms relied on culturing them in the lab, a method that limits the investigation of many species since most are unculturable1. Metagenomics overcomes these issues by allowing the study of microorganisms regardless of their ability to be cultured or the environments they inhabit. Over time, the field has evolved, especially with the advent...-
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